ELISA, IHC, WB
Cytokines & Growth Factors
Conjugated Polyclonal Antibodies
Form & Buffer
Supplied with 0.03% Proclin 300, 50% Glycerol and 0.01M PBS, PH 7.4
Store at 4 deg C for short term storage and -20 deg C for long term storage. Avoid repeat freeze/thaw cycles
RANK antibody (Biotin) was raised in Rabbit using recombinant human Tumor necrosis factor receptor superfamily member 11A protein as the immunogen
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.