TRANCE antibody biotin

FC

Rat

Mouse

IK22/5

Biotin

Blue Ice

anticorps

500 ug/ml

Immunology

IgG2a kappa

Biotinylated

Primary Antibody

FC: ≤ 0.06 ug/test

Conjugated Monoclonal Antibodies

Store at 2-8 deg C. Do not freeze. Protect from light.

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.