Important information

Name

Mouse RANK PicoKine ELISA Kit

Size

1x96 well plate

Catalog number

EK0830

Price

623 €

Extended information

Anti-coagulation

/

Entrez nr.

21934

Minutes of TMB incubation

15-20

Gen protein identity

O35305

ELISA's sensitivity

<2pg/ml

Gene tested

murine RANK

Latin name

Mus musculus

Antigen

NSO, Q30-P213

ELISA kit detection limits

62.5pg/ml to 4000pg/ml

Target of this ELISA

Natural and recombinant mouse RANK

Uniprot

Tumor necrosis factor receptor superfamily member 11A

Gene name

tumor necrosis factor receptor superfamily, member 11a, NFKB activator

ELISA's application

For quantitative measurement of mouse RANK in cell culture supernatant and serum.

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Detector and capure antibodies

A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.006 + 0.109 + 0.205 + 0.432 + 0.719 + 1.184 + 1.659 + 2.038

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);735;1428;2873;873;1740;3226 ELISA standard deviations 38.22;87.11;140.8;57.62;125.28;170.98 CV(%);5.2;6.1;4.9;6.6;7.2;5.3

Alternative

CD265;FEO;LOH18CR1;Loss of heterozygosity 18 chromosomal region 1;ODFR;OFE;OPTB7;Osteoclast differentiation factor receptor;OSTS;PDB2;Receptor activator of NF-KB;Receptor activator of nuclear factor kappa B;TNFRSF11A;TNR11_HUMAN;TRANCER;Tumor necrosis factor receptor superfamily member 11A;Tumor necrosis factor receptor superfamily member 11a activator of NFKB;Tumor necrosis factor receptor superfamily member 11a NFKB activator

How the elisa works

Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse RANK standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant or serum to the ELISA plate. Keep in mind that each mouse RANK standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse RANK antibodies;1 ST0000-10;Lyophilized recombinant mouse RANK standard;10ng/tube×2 AR1107;Biotinylated anti- mouse RANK antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

ELISA kit description

GENTAUR's mouse RANK ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for RANK was used as capure antibodies on the ELISA test plate. The internal standards (NSO, Q30-P213) and your samples are measured with a biotin conjugated polyclonal from goat specific for RANK will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse RANK in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

More gene info

Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.