Minutes of TMB incubation
Gen protein identity
ELISA kit detection limits
62.5pg/ml to 4000pg/ml
Target of this ELISA
Natural and recombinant human RANK
Tumor necrosis factor receptor superfamily member 11A
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
For quantitative measurement of human RANK in cell culture supernatant and serum.
the ELISA does not cross react with any other proteins except from closely related species
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
ELISA standard curve
Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.035 + 0.136 + 0.244 + 0.399 + 0.660 + 1.129 + 1.524 + 2.026
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);655;1863;2533;639;1957;2668 ELISA standard deviations 26.2;68.93;136.8;37.1;90;176.1 CV(%);4;3.7;5.4;5.8;4.6;6.6
CD265;FEO;LOH18CR1;Loss of heterozygosity 18 chromosomal region 1;ODFR;OFE;OPTB7;Osteoclast differentiation factor receptor;OSTS;PDB2;Receptor activator of NF-KB;Receptor activator of nuclear factor kappa B;TNFRSF11A;TNR11_HUMAN;TRANCER;Tumor necrosis factor receptor superfamily member 11A;Tumor necrosis factor receptor superfamily member 11a activator of NFKB;Tumor necrosis factor receptor superfamily member 11a NFKB activator
How the elisa works
Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human RANK standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human RANK standard solution and each sample be measured in duplicate.
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human RANK antibodies;1 ST0000-10;Lyophilized recombinant human RANK standard;10ng/tube×2 AR1107;Biotinylated anti- human RANK antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA kit description
GENTAUR's human RANK ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for RANK was used as capure antibodies on the ELISA test plate. The internal standards (NSO,Q29-G213) and your samples are measured with a biotin conjugated polyclonal from goat specific for RANK will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human RANK in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
More gene info
Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.