Important information

Name

Rat Tumor necrosis factor ligand superfamily member 11 ELISA Kit

Size

96-wells plate (breakable strips)

Catalog number

EK2357

Price

484 €

Extended information

Species reactivity

Rat

Assay procedure

1-4h

Tissue

tumor

Detection wavelengt

450 nm

Latin name

Rattus norvegicus

Applications

Enzyme Linked Immunosorbent Assay

Antigen

Tumor necrosis factor ligand superfamily member 11

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Synonym name

Rattus Tumor necrosis factor ligand superfamily member 11 ELISA Kit

Long name

ELISA test kit for Rat Tumor necrosis factor ligand superfamily member 11

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

General description

This is a ready to use kit for performing ELISA tests for detection of Tumor necrosis factor ligand superfamily member 11 in samples from Rat

Sample types

Our Tumor necrosis factor ligand superfamily member 11 ELISA kit has been proven to work and successfully detect Tumor necrosis factor ligand superfamily member 11 in Rat

Shipping, storage, shelf life

The Tumor necrosis factor ligand superfamily member 11 ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Rat Tumor necrosis factor ligand superfamily member 11 ELISA Kit is 6 months after delivery.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Kit contents

ELISA test kit for Rat Tumor necrosis factor ligand superfamily member 11Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.

Tips

Due to transportation and handling small amounts of the liquid components of the Rattus Tumor necrosis factor ligand superfamily member 11 ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.

Summary of the prodecure

1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.