Important information

Name

Rat OPGL (Osteoprotegerin Ligand) CLIA Kit

Size

96Test

Catalog number

E-CL-R0500-96T

Price

541 €

Extended information

UNIProt ID

NA

Target Synonym

NA

Target Species

Rat

Target Name

OPGL

Type

Sandwich

Sensitivity

3.75pg/mL

Detection Type

Colormetric

Detection Range

6.25~400pg/mL

Latin name

Rattus norvegicus

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Rat OPGL (Osteoprotegerin Ligand) CLIA Kit

Description

FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OPGL . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPGL , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OPGL . You can calculate the concentration of Rat OPGL in the samples by comparing the RLU value of the samples to the standard curve.