Important information

Name

Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein(C-6His) 50µg

Size

50µg

Catalog number

PKSM041165-50µg

Price

577 €

Extended information

Fusion tag

C-6His

Accession

O35235

Mol Mass

28.5 kDa

AP Mol Mass

30-35 kDa

Expressed Host

Human Cells

Group

recombinants

Latin name

Mus musculus

Sequence

Ala73-Asp316

Source

Recombinants or rec. proteins

Purity

>95% as determined by reducing SDS-PAGE.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Activity

Measured by its ability to bind Human TNFRSF11B in functional ELISA.The ED50 for this effect is less than 10 ug/ml.

Synonym

Tumor necrosis factor ligand superfamily member 11;Tnfsf11;Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254

Stability and Storage

Lyophilized protein should be stored at <-20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at <-20℃ for 3 monthshs

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background

Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.