Important information

Name

Recombinant Human RANK L/TRANCE/TNFSF11 Protein(N-6His) 50µg

Size

50µg

Catalog number

PKSH032986-50µg

Price

577 €

Extended information

Fusion tag

N-6His

AP Mol Mass

20 kDa

Accession

O14788

Expressed Host

E.coli

Mol Mass

22.4 kDa

Group

recombinants

Sequence

Ile140-Asp317

Source

Recombinants or rec. proteins

Purity

>90% as determined by reducing SDS-PAGE.

Reconstitution

Please refer to it for detailed information.

Endotoxin

<1.0 EU per µg as determined by LAL test.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Stability and Storage

Lyophilized protein should be stored at <-20℃, though stable at room temperature for 3 weeks

Activity

Measured by its ability to binding SF11A used funtional ELISAThe ED50 for this effect is less than 10 ug/ml.

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Synonym

CD254; ODF; OPGL; RANKL; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.