Soluble RANK Ligand Recombinant Protein

Rat

Q9ESE2

117516

E. coli

16924012

Lyophilized

NP_476490.1

recombinants

Rattus norvegicus

Recombinants or rec. proteins

This product is for research use only.

tumor necrosis factor (ligand) superfamily, member 11

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

RANKL, Opgl, Rankl, Trance, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

Biological activity was determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0 - 10.0 ng/mL.

FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.

PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID

The lyophilized Soluble RANK Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Soluble RANK Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Soluble RANK Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).