Important information

Name

RANK antibody biotin

Size

50 µg

Catalog number

61R-1652

Price

253 €

Extended information

Tested for

FC

Raised in

Rat

Specificity

Mouse

Clone

R12-31

Conjugate/Tag

Biotin

Shipping conditions

Blue Ice

French translation

anticorps

Concentration

500 ug/ml

Isotypes

IgG2a kappa

Conjugation

Biotinylated

Category

Primary Antibody

Usage Recommendations

FC: ≤ 0.25 ug/test

Area of research

Cytokines & Growth Factors

Antibody Subtype

Conjugated Monoclonal Antibodies

Storage

Store at 2-8 deg C. Do not freeze. Protect from light.

Form & Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

Assay Information

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.