Recombinant Human RANK L/TRANCE/TNFSF11 Protein(N-6His)

N-6His

O14788

20 kDa

E.coli

22.4 kDa

recombinants

Ile140-Asp317

Recombinants or rec. proteins

＞90% as determined by reducing SDS-PAGE.

＜1.0 EU per µg as determined by LAL test.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks

Measured by its ability to binding SF11A used funtional ELISAThe ED50 for this effect is less than 10 ug/ml.

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

CD254; ODF; OPGL; RANKL; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.